Highlights
- Fluorescent recombinant allergen tetramers specifically bind to and
activate basophils from patients allergic to bee venom (Api m 1) and
ryegrass pollen (Lol p 1 and Lol p 5).
- Staining intensities of recombinant Api m 1 and Lol p 1 tetramers have
greater positive predictive values for allergy than basophil
activation.
- A single flow cytometric stain with multiple allergen tetramers can be
applied as a rapid component-resolved and differential diagnostic test
for allergy.