2.7 Statistical analysis
All flow cytometry data were analyzed with FACS DIVA v8.0.1 (BD
Biosciences) and FlowJo v10 software packages (FlowJo LLC, Ashland, OR,
US). Statistical analyses were performed using Graphpad Prism (v8.4.1):
the non-parametric Wilcoxon signed rank test for paired data and the
non-parametric Mann-Whitney U-test for unpaired data. Receiver operating
characteristic (ROC) curves were used to determine specificity and
sensitivity of BAT or CytoBas for distinguishing between control and
allergic patients. For all tests, p<0.05 was considered
significant.
RESULTS