FIGURE
6. Multiplex flow cytometry distinguishes between different allergic
sensitizations with a single tube. A) Representative histograms of
[Lol p 1]4, [Lol p 5]4 or [Api
m 1]4 MFI on pDCs (blue) and basophils (red) from
thawed PBMC of control, BV-allergic and RGP-allergic patients. Numbers
accompanying histograms denote MFI (non-bolded indicate pDCs;bolded indicate basophils). Scatterplots depicting correlation
between serum levels of allergen-specific IgE and MFI of allergen
tetramers on basophils stained with B) Api m 1 C) Lol
p 1 and D) Lol p 5 allergen tetramers. AU, arbitrary units.
Significance and r-squared (r2) of correlation
determined by linear regression.