FIGURE 5. Multiplex flow cytometry using multiple allergen tetramers in a single tube as a component-resolved diagnostic test for allergy.A, B) Representative histograms of basophils from a control (blue) or RGP-allergic patient (red) stained with Lol p 1 and Lol p 5 tetramers in a single tube. C) 2D dot plot of Lol p 1 and Lol p 5 tetramer staining of the basophils from the control and patient in panels A and B. D) Scatterplot depicting MFI ratios of allergen:streptavidin for [Lol p 1]4-APC vs strep-APC and [Lol p 5]4-BV711 vs strep-BV711 on basophils from controls (blue, n = 10) and RGP-allergic subjects (red, n=15) stained with both allergen tetramers or both streptavidin conjugates in a single tube.