Abstract
Mungbean
(Vigna radiata [L.])
is
an important
economic
crop grown in South and
East
Asia.
The
low contiguity of the current assembly of V. radiata genome has
limited its application. Here, we report a high-quality chromosome-scale
assembled genome ofV.
radiata to facilitate the investigation of its genome characteristics
and evolution.
By
combination of Nanopore long reads, Illumina short reads and
Hi-C
data, we generated a high-quality genome assembly of V. radiata ,
with 473.67 megabases assembled into 11 chromosomes with contig N50 and
scaffold N50 of 11.3 and 42.4 megabases, respectively. A total of 52.8%
of the genome was annotated as repetitive sequences, among which LTRs
(long terminal repeats) were predominant (33.9%). The genome ofV. radiata was predicted to contain 33,924 genes, 32,470 (95.7%)
of which could be functionally annotated. Evolutionary analysis revealed
an estimated divergence time of V. radiata from its close
relative V. angularis of ~11.66 million years
ago. In addition, 277 V. radiata specific gene families, 18
positively selected genes were detected and functionally annotated.
This
high-quality
mungbean genome will provide valuable resources for further genetic
analysis and crop improvement of mungbean and other legume species.
Running title: High-quality mungbean genome assembly
Keywords:Mungbean
(Vigna radiata ),
Hi-C,
genome
assembly,
genome
annotation