Macroscopic assessment
The extent of depigmentation was evaluated in terms of the percentage of affected area, as described previously, and was scored as follows: 0, no depigmentation (0%); 1.0—10% depigmented area; 2, 10%–25%; 3, 25%–75%; 4, 75%–100%; and 5, 100%. The final vitiligo score of each experimental group was calculated as the average for individual mice.