Toxicity text of phenanthridine derivatives in vivo
To ensure host safety, ICR mice were treated once with HCJA121 and
HCJA404 (intraperitoneally, at a dose of 2000 mg/kg) and subsequently
assessed for 14 days. None of the mice died in the
phenanthridine-treated groups, and there was no change in body weight.
Organs (liver, kidney, testis, and ovary) and blood samples collected at
the end of the experiment were used for pathological and haematological
examination. The results of the pathological analysis are shown in Fig.
S2. No clear abnormalities were observed; however, there was slight
vasodilatation and a low amount of lymphocyte distribution. The white
cells and differential leukocyte parameters (Table S3) and erythrocytic
parameters and platelet level parameters (Table S4) did not clearly
change. It appears that these three compounds exhibit low acute
toxicity; therefore, they can be considered safe according to this in
vivo analysis.