Methods
2S albumin-binding B-cells were single-cell sorted from peripheral blood
of peanut allergic (n=6) and tolerant (n=6) donors sensitized toAra h2 and/or 6 (≥ 0.1 kU/l) and non-atopic controls
(n=5). h 2 and/or 6 (≥ 0.1 kU/l). Corresponding h h eavy
and light chain gene transcripts were heterologously expressed as mAbs
and tested for specificity to native Ara h2 and 6. HCDR3 sequence motifs
were identified by Levenshtein distances and hierarchically clustering.