Conclusion
In this study, we demonstrated that
the FLc-Capture method could
efficiently capture and enrich a
large number of coding and noncoding loci for nonmodel organisms without
any prior genome information. The direct use of homemade full-length
cDNA probes in FLc-Capture can skip the expensive commercial probe
design and synthesis, significantly reducing experimental cost.
FLc-Capture can generate transcriptome-level data just based on DNA
samples, which facilitates including more number of taxa in a
phylogenomic study. In summary, FLc-Capture holds substantial promise in
phylogenomic researches as a universally applicable and cost-effective
sequence capture method of simultaneously collecting genome-level coding
and noncoding orthologous loci for any organism.