STRING networks for pejus and pessimus range physiological states
All treatments 85g/kg and greater represent pessimum range salinities given the ultimate MP endpoint at this salinity. Network analysis was run for the 78 proteins shared by 14-day acclimations and extended salinity treatments at 85g/kg and 105g/kg and compared with the networks for the 87 proteins unique to the extended 75g/kg treatment. Networks were identified with greater than three nodes and classified using ontological term enrichment. Pejus range regulation consisted of a network of mostly upregulated proteins with enrichment in amino acid metabolism and oxidoreductase terms including lactate dehydrogenase (LDH) and glycogen synthase, a network of mixed up and down-regulation of structural proteins, especially myosin subunits, and downregulation of proteasome proteins (Figure 5A). Pessimum range networks contained specific upregulated mitochondrial proteins, with subunits in the cytochrome b-c1 complex as central members, and downregulation of extracellular matrix proteins (ECM) with several collagen subunits (Figure 5B).