Correlation analysis
The ratio of blood osmolality, body condition, time at high salinity
(>65g/kg) and salinity at time of sample collection versus
the control value for each treatment used in the proteomic analysis was
compared against the FC for this treatment was used to compare
treatments based on linear correlation coefficient
(r2). To account for differences between the
short-term and long-term acclimation controls due to the high feed rate
and age difference between these two, the controls were compared using
the same procedure as each treatment to control with MSstats 3.1 and any
proteins which were significantly different between controls were not
considered. Following this filtering step, proteins with
r2 greater than 0.9 were identified and further
refined to only those proteins which were significantly regulated in at
least one treatment. To determine functional groups which were most
correlated with a given parameter, the list of accession numbers of
proteins which met the above conditions were searched on the STRING
database and networks identified.