Blood osmolality
Blood osmolality values were collected for all treatments in the 14-day acclimations and treatments at extended constant salinity (Figure 1C). Blood osmolality was significantly higher (p < 0.05) for all treatments compared with control except 21g/kg. Blood osmolality was 810 mOsm ± 92.6 at MP for the 85g/kg treatment and 797 mOsm ± 107.7 at MP for the 105g/kg treatment. Both treatments had significantly higher (p < 0.05) blood osmolality versus 14-day and 12-week FW handling controls. Blood osmolality was 389 mOsm ± 19.1 following 10 weeks at 75g/kg, and 353.9 mOsm ± 6.38 for the handling control. Following 24 hours after acclimation to 75g/kg in this experiment, blood osmolality was 399 ± 19.2. Acute salinity stress following transfer to 32g/kg had a mean blood osmolality of 655 mOsm ± 20.8 at MP (n=8).