Immunohistochemistry of the RB protein
The detected germline RB1 gene mutation suggested the generation
of a truncated RB protein following translation. The histological
localisation and intracellular distribution of the aberrant protein was
analysed using an anti-RB monoclonal antibody on all samples and on
known positive controls (colorectal adenocarcinoma). While the nuclear
localisation of the RB protein was shown in control slides it was not
detected in the patient samples. RB IHC of the S1, the S3 and the
control was presented in Figure 2 .