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Figure 1. Conventional histological (H&E) characteristics of patient tumor samples. A : Rb of the right eye bulb, B : chondroblastic osteosarcoma of the left orbital bone, C : osteoblastic osteosarcoma from the left tibia, D : osteoblastic osteosarcoma, pulmonary metastasis. Arrows indicate retinoblasts, as well as chondroblastic and osteoblastic propagation (magnification: x20).
Figure 2. Immunohistochemistry of RB protein. A : retinoblastoma (S1), B : left orbital bone (S3), C : colorectal adenocarcinoma control. Arrows indicate the IHC-negative cell nuclei of retinoblastic/ chondroblastic tumors and the positive nuclear staining in adenocarcinoma cells (magnification: x40).
Figure 3. Structure and interactions of RB1. Schematic represenation of full-length RB1 protein is shown. The structures of N-terminal and central regions are represented, and the protein-protein interactions of C-terminal region are also shown based on structural data. Sequence of the C-terminal of region of RB1 is shown by black color. Blue arrows show position of p.Gln850Ter mutation. The sequences that are involved in protein-protein interactions are shown for each coordinate file, these regions are highlighted by red in the complex structures, as well.