DNA isolation
DNA isolation from peripherial blood was performed using QIAamp DNA Mini
Kit (Qiagen, Hilden, Germany). Genomic DNA was extracted from FFPE
tissues using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany).
The isolations were carried out according to the manufacturer’s standard
protocol and the DNA was eluted in 50 µL elution buffer. The DNA
concentration was measured in the Qubit dsDNA HS Assay Kit using a Qubit
4.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).