Harvest
We harvested the plants after eight weeks and separated the shoot from the root by cutting the stem just below the rosette formation. We stored the shoots in paper bags and dried the material at 70 ˚C. We washed the sand from the roots and homogenized them. We weighed root material, and took subsamples for DNA isolation. We stored the subsamples at -20 ˚C and dried the remaining roots material in paper bags at 70 ˚C.
In-vitro root organ cultures