Harvest
We harvested the plants after eight weeks and separated the shoot from
the root by cutting the stem just below the rosette formation. We stored
the shoots in paper bags and dried the material at 70 ˚C. We washed the
sand from the roots and homogenized them. We weighed root material, and
took subsamples for DNA isolation. We stored the subsamples at -20 ˚C
and dried the remaining roots material in paper bags at 70 ˚C.
In-vitro root
organ cultures