Enzyme assay and anti-mycobacterial screening
The enzymatic activity of MTSK was assessed through ADP-Glo kinase assay as mentioned the previous study.7For inhibition assay, MTSK was incubated at different concentrations (12.5µg/ml to 50µg/ml) of compounds for an hour at 37°C, subsequently followed by addition of 2µg/ml shikimic acid. After completion of overall reaction, phosphorylation of substrate by the kinase was measured by luminometer in terms of relative luminescence unit (RLU).Anti-mycobacterial screening was done using MABA and MGIT according to our previous study.7, 8