Comparative domain analysis of SK of different mycobacterial
strains
Clustal Omega pairwise sequence alignment software was used to analyse
the domains of SK of H37Rv and M.smegmatis (MS). It was observed
that CD was the only domain which is100% conserved in both the strains
(Figure 2A). Using MABA MIC
ofS-014-1049was calculated against
H37Rv, H37Ra and MS. The three strains showed inhibition at a
concentration of 3.12µg/ml,
12.5µg/ml and 25µg/ml respectively (Figure 2B). Since, all the three
strains have a conserved CD and are inhibited by the compound which
possibly suggests thatS-014-1049 binds to CD of SK.