Effect of S-014-1049 on Ra-wt at different concentrations
Log phase culture of Ra-wt was taken and treated with S-014-1049 and
rottlerin at 1µg/ml, 2µg/ml and 4µg/ml for an hour with constant
shaking. After treatment lysates of the culture were prepared and
analysed using western blotting. Maximum inhibition of Ra-wt was
observed at 2X MIC concentration of S-014-1049 whereas rottlerin showed
significant inhibition at all concentrations (Figure 1E, F).