Inhibitory profiling of S-014-1049 against mutant SK
Comparative study of the kinase activity of the 3 mutants (CD15:SK, SBD34:SK, and LD117:SK) of SK using ADP-Glo kinase assay and wild type MTSK was used as a control. Significant inhibition of enzyme activity was observed in SBD34:SK, LD117:SK and MTSK compared to CD15:SK. Hence, it can be inferred that the binding of the compound with the protein, occurs in case of intact CD which influences in the decrease of enzyme activity (Figure 2E).