Comparative domain analysis of SK of different mycobacterial strains
Clustal Omega pairwise sequence alignment software was used to analyse the domains of SK of H37Rv and M.smegmatis (MS). It was observed that CD was the only domain which is100% conserved in both the strains (Figure 2A). Using MABA MIC ofS-014-1049was calculated against H37Rv, H37Ra and MS. The three strains showed inhibition at a concentration of 3.12µg/ml, 12.5µg/ml and 25µg/ml respectively (Figure 2B). Since, all the three strains have a conserved CD and are inhibited by the compound which possibly suggests thatS-014-1049 binds to CD of SK.