Enzyme assay and anti-mycobacterial screening
The enzymatic activity of MTSK was assessed through ADP-Glo kinase assay
as mentioned the previous study.7For inhibition assay,
MTSK was incubated at different concentrations (12.5µg/ml to 50µg/ml) of
compounds for an hour at 37°C, subsequently followed by addition of
2µg/ml shikimic acid. After completion of overall reaction,
phosphorylation of substrate by the kinase was measured by luminometer
in terms of relative luminescence unit (RLU).Anti-mycobacterial
screening was done using MABA and MGIT according to our previous
study.7, 8