2.3. Mitochondrial genome annotation
These two mtDNAs were assembled manually, and annotated preliminarily by
Geseq
(https://chlorobox.mpimp-golm.mpg.de/geseq.html)
with the reference of related species, T. pisiformis and T.
crassiceps , identified by the cox 1 gene alignment of
Neighbor-Joining method in MEGA 5.1 (data not show). Putative tRNA genes
were identified using ARWEN
(http://130.235.46.10/ARWEN/)
(Laslett & Canbäck, 2008). The positions of their open reading frames
and rRNA genes were also further checked and modified based on alignment
with the mt genomes annotation of T. pisiformis and T.
crassiceps , respectively. SnapGene (v3.2.1) was used to translate the
amino acid sequence of the protein-coding genes with genetic code of the
Echinoderm, Flatworm Mitochondrial and map the annular diagram of the mt
genomes.