2.5 Validation by cultivation with adding important components
To validate the estimation by DNN, E. coli EmGFP were cultivated in the basal medium containing 0.05 g/L of an important component as estimated by DNN. The experiments were performed in triplicate. The yield of cells and GFP were evaluated after 9 h of cultivation, and the yield fold changes were calculated by normalizing these yields in reference to the control cultivation. The significance of these values was evaluated by F-tests and T-tests (p <0.05).