3.5 Validation by supplemental cultivation
We performed validation cultivations to confirm the estimation of
important components by DNN-MIE. Figure 5 demonstrates the
results of these validation experiments. Glu, maltose, Alanine (Ala),
phenylalanine (Phe), Ile, trehalose, Lys, Asp, phosphate, Gly, and
sorbitol significantly increased GFP yields, and leucine (Leu), Serine
(Ser), threonine (Thr), asparagine (Asn), valine (Val), glycerol, and
tyrosine (Tyr) significantly decreased GFP yields. In particular,
because Glu increased the GFP yields to 112.9 ± 4.0% and the cell
yields to 104.8 ± 1.5%, this amino acid was predicted to be the most
important variable. Maltose stimulated the GFP yields by 106.5 ± 1.2%
and the cell yields by 104.9 ± 1.1%. Asp induced less than 2% of the
cell and GFP yields. Sorbitol showed no influence on the cell and GFP
yields. The final pH was between 6.44 and 6.54 in all cases.