Figure 4.- Therapeutic effect of BEA in TB and T2D / TB mice.Groups of TB and T2D/TB mice after 60 days of intratracheal infection with high dose Mtb strain H37Rv were treated during one or two months with BEA, while the control group received only the vehicle. Lungs were processed for the indicated determination. In comparison with the control mice, in both TB and T2D/TB groups and particularly after 60 days of treatment, BEA induced significant decrease of pulmonary bacillary loads and tissue damage (pneumonia), while after one month of treatment BEA induced higher expression of the protective cytokines TNFα and IFN-γ. In the spleen, treatment with BEA did not produce significant changes in the weight and CFUs in TB mice, while in T2D/TB mice induced after two months of treatment significant decrease of bacillary burdens and higher weight increase. Asterisks represent statistical significance (**** p< 0.0001, two- way ANOVA and n.s. no significance).