Figure 5.- Effect of BEA on lung glucocorticoids production and the gene expression of their converting enzymes in TB and T2D-TB mice.A) Representative low power and mild power micrographs of immunohistochemistry detection of corticosterone, in both groups after one and two months of BEA treatment. B) In comparison with the control group, the digital pathology analysis showed significant lower immunostaining of corticosterone induced by BEA treatment after one and two months in TB mice and T2D-TB mice. C) BEA treatment induced lower 11b-HSD1 gene expression in TB and T2D-TB in comparison with vehicle-treated animals. D) Representative micrographs of cortisone detection by immunohistochemistry in the lungs of TB and T2D mice treated with BEA at one and two months of infection. E) The digital pathology study shows higher expression of cortisone after one month of treatment in the TB and T2D/TB groups. F) Gene expression of 11b-HSD2 corticosterone converting enzyme after BEA treatment in TB and T2D-TB animals. Asterisk represents statistical significance (p<0.05).