Materials and methods
In this study, no regular pathogens causing piglet diarrhea were
detected in feces and drinking water samples of diarrheal piglets
collected in farm areas where diarrhea occurred, but a β-hemolytic
bacterium was found, which was also isolated in the drinking water.
Subsequently, 16SrRNA primers were used to amplify and sequence, and the
results showed that the bacteria were a C. haemolyticum like
bacterium by phonotype. The bacteria isolated were used for the
challenge trial. Nine 30-day-old healthy piglets were randomly divided
into three groups. 2ml of the 3.5x10^9 colony forming unit (CFU) of
the bacterium were administered orally or intramuscularly injected
respectively, and 2ml of PBS was administered orally to the control
group. The isolate GDHYZ30 could cause the same diarrhea symptoms
similar to that of the clinical cases when the piglets were challenged.
To understand the genetic characteristics of the bacteria, whole gene
sequencing was Completed by Sangon Biotech Company. This whole-genome
sequencing project has been deposited at GenBank under the accession
number: PRJNA802706.