2.4. Statistical analysis.
After testing the normality of distribution data (Shapiro-Wilk test) and homogeneity of variance, unpaired Student’s t test, Welch’s t test, Mann-Whitney U test were used to analyse gene expression, ELISAs and western blot results. Statistical analysis was performed using GraphPad Prism 9 software (San Diego, USA) or IBM SPSS statistics v24 software. For the behavioural data, we used the ANOVA for repeated measures followed by Bonferroni multiple comparisons as post hoc test when appropriate. Results are expressed as mean ± SEM and the statistical significance was set at p < 0.05. All the p values of the statistic tests are reported in Table 1 and the p values from thepost hoc analysis are described in the figure legends.