2.2.2.1. Behavioural experiment protocol.
To study the influence of KOR/DYN system in pain-induced negative affective states in NAc of female rats, the same protocol of experiment 1 was used except for the additional bilateral infusion of the kappa opioid receptor antagonist norbinaltorphimine (NorBNI) into the NAc medial shell. The week prior to the CFA administration after the basal measures for VFT and SPT were obtained, animals were injected with NorBNI or artificial cerebral spinal fluid (aCSF) into the NAc medial shell. The following 14 days after CFA administration animals were tested following the VFT, LDB and SPT paradigms. The timeline followed is summarised in figure 4A and figure S3A.