Protein extraction.
For protein analysis, we used a previous published protocol of protein extraction (Lorente et al., 2022). 0.5 mL of cold lysis buffer (1% IGEPAL CA-630, 20mMTris-HCl pH 8, 130MNaCl, 10mMNaF, and 1% proteases inhibitor cocktail [Sigma, Darmstad, Germany]) was used to homogenate 250 mg of brain tissue, thus the volume of the lysis buffer was adapted to the real quantity of tissue in each sample. Then we maintained the samples in ice for 30 minutes, before centrifugate at 13000 G for 15 minutes at 4 ยบ C and collect the supernatant. Furthermore, we determined the concentration of the lysates by using a Bradford protein assay kit (Bio-Rad).