2.4. Statistical analysis.
After testing the normality of distribution data (Shapiro-Wilk test) and
homogeneity of variance, unpaired Student’s t test, Welch’s t test,
Mann-Whitney U test were used to analyse gene expression, ELISAs and
western blot results. Statistical analysis was performed using GraphPad
Prism 9 software (San Diego, USA) or IBM SPSS statistics v24 software.
For the behavioural data, we used the ANOVA for repeated measures
followed by Bonferroni multiple comparisons as post hoc test when
appropriate. Results are expressed as mean ± SEM and the statistical
significance was set at p < 0.05. All the p values of the
statistic tests are reported in Table 1 and the p values from thepost hoc analysis are described in the figure legends.