2.2.2.1. Behavioural experiment protocol.
To study the influence of KOR/DYN system in pain-induced negative
affective states in NAc of female rats, the same protocol of experiment
1 was used except for the additional bilateral infusion of the kappa
opioid receptor antagonist norbinaltorphimine (NorBNI) into the NAc
medial shell. The week prior to the CFA administration after the basal
measures for VFT and SPT were obtained, animals were injected with
NorBNI or artificial cerebral spinal fluid (aCSF) into the NAc medial
shell. The following 14 days after CFA administration animals were
tested following the VFT, LDB and SPT paradigms. The timeline followed
is summarised in figure 4A and figure S3A.