Von Frey test.
The VFT was used to assess the mechanical nociceptive thresholds before (basal) and after the CFA injection. After 5-10 min of habituation to the apparatus, Von Frey filaments (Aesthesio®) were manually applied to the injected hind paw with the simplified up-down method (Bonin et al., 2014). Results were expressed as the mean ± SEM of mechanical sensitivity threshold (in grams, g).