2.2.1.1. Behavioural experiments design and brain collection.
To study the time course and the gender effect of negative affective states induced by inflammatory pain we have selected a combination of different behavioural approaches. One week prior to CFA administration, we obtained basal nociceptive measures by means of Von Frey test (VFT) together with basal measures of the sucrose preference test (SPT). During the following 18 days after complete Freund Adjuvant (CFA) administration, animals were repetitively tested in the VFT, light-dark box test (LDB) and SPT (n=12-14/group). Finally, 18 days after the CFA administration, rats were sacrificed to collect their brains. Details of this protocol and timeline are summarized in figure 1A and figure S1.