2.2.2.3. Microinjection placement assessment.
At the end of the protocol, rats were sacrificed under isoflurane
overdose and brains were removed and rapidly frozen in dry ice; 40
µm-thick coronal slices of the NAc shell were obtained using a cryostat
(Micron, Boise, USA) and stained with a cresyl violet (Sigma, St. Louis,
USA) protocol to verify proper probe placement as we have used
previously (Campos-Jurado et al., 2020). Only animals that received a
bilateral injection into the NAc medial shell were included in the
analysis (figure S3B).