Rice materials and M. oryzae strains
Nipponbare (Oryza sativa L. ssp. japonica cv.) and Zhonghua11 (Oryza sativa L. ssp. japonica cv.) were kept in the Rice Research Institute, Fujian Academy of Agricultural Sciences. Suxiu867, a japonica cultivar, was used as the recipient, and Minghui86, a restorer indica cultivar, was used as the donor. The F1 plants were generated from Suxiu867 as female and Minghui86 as male. The F1 plants were back crossed with Suxiu867 to produce the BC1F1generation. These BC1F1 plants were backcrossed to Suxiu867 to produce BC2F1, and these plants were self-pollinated to produce BC2F2 lines. These lines were self-interbred for 6 generations, and a stable line was obtained, named as Hui1586. Rice plants were grown in the natural field or in the green house at 28 C under 12-h light and 12-h dark condition.
The M. oryzae isolates Guy11, 18SH-D527, FJ2011, 95085AZB and 18NH-16-3 were kindly provided by the State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Fujian Agriculture and Forestry University; isolate Zhong 1 was provided by Sichuan Agricultural University; isolates KJ201, 501-3, RB22 and 20-15 were from Fujian Academy of Agricultural Sciences and isolates M409, MH86-1, and MH86-3 have been isolated and maintained at the Plant Immunity Center, Fujian Agriculture and Forestry University.