Rapid and dramatic transcription reprograming occurs in the
resistant rice cultivar Hui1586 challenged with M. oryzae
To gain more details about the M. oryzae-induced DEGs in
Nipponbare and Hui1586, we compared DEG overlaps between them at each
time point. Large DEG overlaps, especially upregulated DEGs, were
observed between the two cultivars at all four time points (Fig. 4A),
indicating that a large number of common genes were involved in the
transcriptional reprograming between the susceptible and resistant
cultivars. As the early time point is crucial for determining the
interactions between plant and pathogens (Mine et al., 2018), we
performed hierarchical clustering of the DEGs identified at 12 hpi in
the two cultivars based on the log2-transformed
fold-change value (Supplemental dataset 4). This analysis generated a
global view of the expression levels of those DEGs at four time points
in Nipponbare and Hui1586. As shown in Fig. 4B, a large portion of DEGs
was induced more strongly at 12 h in Hui1586 compared with Nipponbare,
suggesting that a faster and stronger activation of transcriptional
reprogramming is important for rice efficient defense against M.
oryzae .