Rice materials and M. oryzae strains
Nipponbare (Oryza sativa L. ssp. japonica cv.) and
Zhonghua11 (Oryza sativa L. ssp. japonica cv.) were kept
in the Rice Research Institute, Fujian Academy of Agricultural Sciences.
Suxiu867, a japonica cultivar, was used as the recipient, and
Minghui86, a restorer indica cultivar, was used as the donor. The
F1 plants were generated from Suxiu867 as female and
Minghui86 as male. The F1 plants were back crossed with
Suxiu867 to produce the BC1F1generation. These BC1F1 plants were
backcrossed to Suxiu867 to produce
BC2F1, and these plants were
self-pollinated to produce BC2F2 lines.
These lines were self-interbred for 6 generations, and a stable line was
obtained, named as Hui1586. Rice plants were grown in the natural field
or in the green house at 28 ◦C under 12-h light and
12-h dark condition.
The M. oryzae isolates Guy11, 18SH-D527, FJ2011, 95085AZB and
18NH-16-3 were kindly provided by the State Key Laboratory for
Ecological Pest Control of Fujian and Taiwan Crops, Fujian Agriculture
and Forestry University; isolate Zhong 1 was provided by Sichuan
Agricultural University; isolates KJ201, 501-3, RB22 and 20-15 were from
Fujian Academy of Agricultural Sciences and isolates M409, MH86-1, and
MH86-3 have been isolated and maintained at the Plant Immunity Center,
Fujian Agriculture and Forestry University.