Energy metabolism pathways were strongly suppressed by M.
oryzae on susceptible rice
KEGG pathway enrichment analysis of M. oryzae- upregulated genes
at 12, 24, 36, or 48 hpi in both Nipponbare and Hui1586 revealed that
immunity-related pathway terms, such as “Diterpenoid biosynthesis”,
“Flavonoid biosynthesis”, “Plant-pathogen interaction”, “MAPK
signaling pathway” and “Phenylpropanoid biosynthesis”, were
significantly enriched (Fig. S1B and Supplemental table 1), indicating
the immune systems were activated in both susceptible and resistant rice
cultivar in response to M. oryzae infection. On the contrary,
energy metabolism pathways, such as “Photosynthesis”, “Photosynthesis
– antenna proteins” or “Carbon fixation in photosynthetic organisms”
were enriched in the M. oryzae- suppressed genes on both cultivar
at 12, 36 and 48 hpi (Fig. S1A and Supplemental table 1). The result is
consistent with the trade-off between immunity and growth (Huot, Yao,
Montgomery, & He, 2014; Smakowska, Kong, Busch, & Belkhadir, 2016).
Notably, the “Photosynthesis” pathways were enriched in the Nipponbare
specific, M. oryzae down regulated DEGs at 24, 36 and 48 hpi, but
not in the Hui1586 specific DEGs, suggesting that Energy metabolism
pathways were strongly suppressed by M. oryzae on susceptible
rice.