Energy metabolism pathways were strongly suppressed by M. oryzae on susceptible rice
KEGG pathway enrichment analysis of M. oryzae- upregulated genes at 12, 24, 36, or 48 hpi in both Nipponbare and Hui1586 revealed that immunity-related pathway terms, such as “Diterpenoid biosynthesis”, “Flavonoid biosynthesis”, “Plant-pathogen interaction”, “MAPK signaling pathway” and “Phenylpropanoid biosynthesis”, were significantly enriched (Fig. S1B and Supplemental table 1), indicating the immune systems were activated in both susceptible and resistant rice cultivar in response to M. oryzae infection. On the contrary, energy metabolism pathways, such as “Photosynthesis”, “Photosynthesis – antenna proteins” or “Carbon fixation in photosynthetic organisms” were enriched in the M. oryzae- suppressed genes on both cultivar at 12, 36 and 48 hpi (Fig. S1A and Supplemental table 1). The result is consistent with the trade-off between immunity and growth (Huot, Yao, Montgomery, & He, 2014; Smakowska, Kong, Busch, & Belkhadir, 2016). Notably, the “Photosynthesis” pathways were enriched in the Nipponbare specific, M. oryzae down regulated DEGs at 24, 36 and 48 hpi, but not in the Hui1586 specific DEGs, suggesting that Energy metabolism pathways were strongly suppressed by M. oryzae on susceptible rice.