Exercise provocation tests
There were 20 CM allergy patients who developed allergic symptoms at the first EPT (Figure 1). One CM-EPT-negative patient experienced a convincing event of EIARD at home. Therefore, 21 patients (49%) were diagnosed as EIARD-positive, and 22 patients were EIARD-negative for CM. Table S1 shows the characteristics and the results of EPTs of the 21 CM EIARD-positive patients. There were eight (38%) patients who were administered antihistamines and eight (38%) patients who were administered β2 stimulants, of whom one received both antihistamines and β2 stimulants at the first EPT. Two patients received an intramuscular injection of adrenaline for anaphylactic shock (cases 17 and 19). Of these 21 CM EIARD-positive patients, seven (33%) underwent a second EPT, of whom four tested negative (Figure 1). The second EPT was performed within a median of 1.5 (range: 0.9–3.4) years after the first EPT.
Among the 31 patients with a wheat allergy, 14 patients were EPT-positive (Figure 2). One wheat-EPT-negative patient experienced EIARD at home. Thus, 15 patients (48%) were diagnosed to be EIARD-positive and 16 patients were EIARD-negative for wheat. Table S2 shows the characteristics of the 15 wheat EIARD-positive patients. There were seven (47%) patients who were administered antihistamines and six (40%) patients who were administered β2 stimulants, of whom five received both antihistamines and β2 stimulants at the first EPT. None of the patients received adrenaline. In total, six (40%) patients who tested positive during the first EPT underwent a second EPT after a median of 2.1 (range: 0.4–5.9) years. Of these, four tested negative (Figure 2).