2.16 Data and statistical analysis
The data and statistical analysis comply with the recommendations of theBritish Journal of Pharmacology on experimental design and
analysis in pharmacology (Curtis et al., 2018). All data in the text,
tables, and figures are presented as means ± SD or means ± SEM.
Statistical analysis was performed using GraphPad Prism 5 (GraphPad
Software, Inc., La Jolla, CA, USA, RRID:SCR_002798). Statistical
significance was determined by using two-way ANOVA or unpaired
two-tailed Student’s t -test. P values < 0.05
were considered statistically significant. Representative data are
presented as raw data. Unless indicated otherwise, five or more
independent experiments were performed. Blinding or randomization was
undertaken in this study.