FIGURE LEGENDS
FIGURE 1Effects of soil microorganisms on
plant fitness (mean ± SE, n = 3). The following metrics were used for
this comparison: (a) biomass accumulation, (b) allocation patterns, and
(c) leaf dry mass per unit area (LMA) of N. tabacum grown in
continuous cropping soil (CCS) and natural fallow soil (FS).
Significance values of the
factorial analysis (ANOVA) for the effects are indicated as follows: *,p < 0.05; **, p < 0.01. Filled (or
open) points show soil without (or with)
sterilization treatment.
FIGURE 2Overview of the taxonomy of
bacterial diversity on phylum level in (a) bulk soil and (b)
rhizosphere
of CCS and FS.
FIGURE 3 Principal
coordinate analysis (PCoA) using Bray-Curtis distance between samples
for taxonomic profiles of bacteria and fungi (operational taxonomic unit
(OUT) level for the 16S rRNA data set) for bulk soils and rhizosphere
of CCS and FS. Permutational
multivariate analysis of variance (PERMANOVA) was used to assess group
differences.
FIGURE 4 Significantly
retrieved discriminant taxa for bulk soil and rhizosphere bacterial and
fungi communities of CCS and FS. The cladogram provides a taxonomic
representation of statistically consistent differences between
rhizosphere and bulk soil for (a) bacterial communities, (b) fungal
communities, and (c) bacterial communities of the reassembled soil
microbial communities. The tables below each cladogram report
phyla/classes that statistically significantly discriminated bulk soilvs rhizosphere. Filter criteria: for bacterial microbiomes: LDA
score > 4.0, p < 0.05; for fungal
microbiomes: LDA score > 3.0, p < 0.05.