SUPPLEMENTARY INFORMATION
Supplementary FIGURE S1 Rarefaction and Goods’s coverage index. Rarefactions were assessed and all samples had a coverage of more than 90% (Subsampling: bacteria (11 000 reads): > 93%; fungi (10 000 reads): > 98%).
Supplementary FIGURE S2 Alpha-diversity indexes of Sobs and Ace for bulk soil and rhizosphere (a, b) bacterial and (c, d) fungal communities of CCS and FS, respectively. Significance values of the Welch’s t-test for the effects are indicated as follows: *, p < 0.05; **, p< 0.01.
Supplementary FIGURE S3 Overview of the taxonomy of fungal diversity on class level in (a) bulk soil and (b) rhizosphere of CCS and FS.
Supplementary FIGURE S4 Pie plots show the core microbiomes in rhizosphere of tobacco. Bacterial and fungal OTUs were classified up to genus level, respectively. p: phylum; c: class; o: order; f: family; g: genus.
Supplementary TABLE S1Taxon analysis on bacterial communities of 24 samples. C_NS: continuous cropping soil without sterilization treatment; C_S: continuous cropping soil with sterilization treatment; F_NS: natural fallow soil without sterilization treatment; and F_S: natural fallow soil with sterilization treatment.
Supplementary TABLE S2 Taxon analysis on fungal communities of 24 samples.
Supplementary TABLE S3 Linear discriminant analysis (LDA score > 4.0, p < 0.05) effect size (LEfSe) of bacterial species in bulk soil and rhizosphere in the reassembled soil microbial communities of CCS and FS.
Supplementary TABLE S4 The core microbiomes in rhizosphere of tobacco found in continuous cropping soil (CCS) and natural fallow soil (FS).
Supplementary TABLE S5Effects of continuous tobacco cropping practice on rhizosphere effect. Microbial species exclusively enriched in bulk soil or rhizosphere. Species numbers over 20% of the total numbers were indicated in bold. “/”: total species numbers less than 10 were not counted.
Supplementary TABLE S6 Effects of natural fallow practice on rhizosphere effect. Microbial species exclusively enriched in bulk soil or rhizosphere. Species numbers over 20% of the total numbers were indicated in bold. “/”: total species numbers less than 10 were not counted.