Flow Cytometry Analysis
Cells transfected with pEF-EGFP were analyzed 24 or 48 hours following transfection to determine the percentage of transfected cells (%EGFP+) and EGFP expression levels (mean EGFP) using either a FACSCalibur Flow Cytometer (Figure 1) or a Millipore Sigma Guava easyCyte HT sampling Flow Cytometer (Figures 2-6). Cells were analyzed via side scatter analysis to differentiate live cell populations from cell debris, while gates for fluorescent (EGFP+) populations were defined using non-transfected (EGFP-) control samples.