Plasmid/Promoter Cloning
Initial screening experiments for the comparison of different vehicles in Jurkat cells used either the luciferase (luc2 gene)30 expression plasmid pGL4.50 (Promega, #E1310) or the EGFP expression plasmid pEF-GFP (Addgene, #11154). The pEF-Luc plasmid used in Figures 1 & 2 was prepared by replacing the EGFP gene in pEF-GFP with the luc2 gene. For the promoter comparison experiments in Figure 2, the EF1α promoter in pEF-Luc was replaced with the CAG promoter (cloned from Addgene plasmid #48138) and the CMV promoter (cloned from pGL4.50).