This study was supported by the National Natural Science Foundation of China (31700302).

Altair, B.S., Kristi, A. K., Marcelo, E. R., George, E. B., Jeferson, A. B., Ana, C.A S., Marcos, A. B.,2018.Hair in Parkinson’s disease patients exhibits differences in Calcium, Iron and Zinc concentrations measured by flame atomic absorption spectrometry-FAAS, J. Trace Elem. Med. Bio,. 47, 134-139.

Asghar, T., Jamil,Y., Iqbal,M., Haq,Z.U., Abbas,M.,2016. Laser light and magnetic field stimulation effect on biochemical, enzymes activities and chlorophyll contents in soybean seeds and seedlings during early growth stages, J. Photoch. Photobio. B, 165, 283-290.

Berni,R., Luyckx,M., Xu,X., Legay,S., Sergeant,K., Hausman,J.F., Lutts,S., Cai,G., Guerriero G.,2019. Reactive oxygen species and heavy metal stress in plants: Impact on the cell wall and secondary metabolism, Environ. Exp. Bot., 161, 98-106.

Carolina, W., Ribeiro, Ana, Paula, K., Julio, A. G., Douglas, J.M., Fabricio, E.L., Carvalho, R.H.V. ,Sousa, A.C., Felipe, K., Teixeira, J.A.G., Silveira, M.M.,2017. Rice peroxisomal ascorbate peroxidase knockdown affects ROS signaling and triggers early leaf senescence, Plant Sci., 263, 55-65.

Chen,L.W., Yang,S.J., Huang,Y., Zhang,B.G., Kang,F.X., Ding, D., Cai,T.M.,2019. Degradation of antibiotics in multi-component systems with novel ternary AgBr/Ag3PO4@natural hematite heterojunction photocatalyst under simulated solar light, J. Hazard. Mater., 371, 566-575.

Fan,Y.J., Yu,Q.S., Wang, G., Tan,J.W., Liu, S., Pu,S.R., Chen,W.C., Xie, P., Zhang,Y.X., Zhang,J., Liao,Y.X., Luo,A.X.,2020. Effects of non-thermal plasma treatment on the polysaccharide from Dendrobium nobile Lindl. And its immune activities in vitro, Int. J. Biol. Macromol., 153, 942-950.

Gao,J.Q., Liu,L., Ma,N., Yang,Jiao., Dong,Z., Zhang,J.S., Zhang,J.L., Cai M.,2020. Effect of ammonia stress on carbon metabolism in tolerant aquatic plant-Myriophyllum aquaticum,Environ. Pollut., 263, 114412.

Gupta, D. K.，Vandenhove, H.，Inouhe, M.,2013.Role of phytochelatins in heavy metal stress and detoxification mechanisms in plants. Heavy Metal Stress in Plants，1 (1),73-94．https://doi.org/10.1007/978-3-642-38469-1_4

Jia, L.,　Yu, K.X., Yao, L.T., Wen, F.J.,2004. Analysis of Organized Degree and Content of Amino Acid in the Zn-accumulation Mycelia of Cordyceps sinensis. Food and Fementation industries, 30(11),94-98. (In Chinese)

Jin, L., Gao, M.J., Duan, X.H., Guo, X.R.,2015. Characteristics of zinc accumulation. in different parts of Catharanthus roseus under Zinc Stress regulated by exogenous Ethrel . Plant research, 35 (01),150-153. (In Chinese)

Jin, L., Gao, M.J., Duan, X.H., Guo, X.R.,2015. Zn Stress Changchun Spend Different Parts of Zn Accumulation by Exogenous Ethephon Regulation. Bulletin of Botanical Research, 35(1): 150 -153. DOI:10.7525/j.issn.1673-5102.2015.01.023

Kunpatee, K., Traipop, S., Chailapakul,O., Chuanuwatanakul, S.,2020. Simultaneous. determination of ascorbic acid, dopamine, and uric acid using graphene quantum dots/ionic liquid modified screen-printed carbon electrode, Sensor Actuat. B- Chem, 314,128059.

Li, Y.Q., Kong, D.X., Fu,Y., Sussman, M.R., Wu Hong.,2020. The effect of. developmental and environmental factors on secondary metabolites in medicinal plants, Plant Physiol. Bioch., 148, 80-89.

Li, C., Huang,Q., Xiao,J., Fu,Xi., You,L., Liu,R.H.,2016. Preparation of Prunella. vulgaris polysaccharide-zinc complex and its antiproliferative activity in HepG2 cells, Int. J. Biol. Macromol., 91, 671-679.

Liao, W.Z., Lu, Y.J, Fu, J.N, Ning, Z.X, Yang, J.G, Ren, J.Y.,2015. Preparation and. Characterization of Dictyophora indusiata Polysaccharide–Zinc Complex and Its Augmented Antiproliferative Activity on Human Cancer Cells. J. Agr. Food. Chem., 63(29) ,6525-6534.

Liu, F., Chen, G.T., Hu, Q.H., Zhao, S.W., Zhao, L.Y.,2014. Separation, Purification and Structure Characteristics of Zn-Binding Polysaccharides from Flammulina velutip. Food Science. 35(5),1-7. (In Chinese)

Liu,H., Jiang,W.B., Cao,J.K., Li,Y.C.,2019. Changes in Extractable and Non-extractable Polyphenols and Their Antioxidant Properties during Fruit On-tree Ripening in Five Peach Cultivars, Hortic. Plant J., 5( 4), 137-144.

Luo, A., and Fan, Y.J,2011. In vitro Antioxidant of a Water-Soluble Polysaccharide from Dendrobium fimhriatum Hook.var.oculatum Hook. Int. J. Mol. Sci. 12, 4068-4079.

Luo, A.X., He, X.J., Zhou, S.D., Fan, Y.J., Luo, A.X, Chun, Z.,2010. Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl. Carbohyd Polym. 79,1014-1019.

Milosevic, N , Slusarenko, A. J.,1996. Active oxygen metabolism and lignification in the hypersensitive response in bean. Physiol. Mol. Plant P., 49(3),143-158.

Nagalakshmi, N , Prasad, M. N. V., 2001. Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus. Plant Sci., 160(2),0-299.

Nie,X.Q., Chen,Y., Li,W., Lu,Y.L.,2020. Anti-aging properties of Dendrobium nobile Lindl.: From molecular mechanisms to potential treatments, J. Ethnopharmacol., 257,112839

Park, J. Y., Song, W.Y., Donghwi, ko., 2012. The phytochelatin transporters AtABCC1. and AtABCC2 mediate tolerance to cadmium and mercury. Plant J, (69), 278-288.

Park,S.J., Ahn,Y.J.,2016. Multi-walled carbon nanotubes and silver nanoparticles differentially affect seed germination, chlorophyll content, and hydrogen peroxide accumulation in carrot (Daucus carota L.), Biocatal. Agr. Biotechnol., 8,257-262.

Quy, D.D., Artik, E.A., Phuong, L.T., Lien, H.H., Felycia, E.S., Suryadi, I., Yi,H.J.,2014.Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica, J. Food Drug. Anal., 22(3), 296-302.

Rama, D., Rai, J.P.N., 2010.Phytochelatins: peptides involved in heavy metal detoxification. Appl. Biochem. Biotech., 160, 945-963.

Rana, S. V.,1996. Protective effects of GSH, vitamin E, and selenium on lipid peroxidation in cadmium-fed rats. Biol. Trace Elem. Res., 51,161-168.

Rai, L.C., Gaur, J.P., Kumar, H.D.,1981. Protective effects of certain environmental factors on the toxicity of Zn, merecury and methylmercury to chlorella vulgaris. Environ Res, 25,250-259.

Salt, D. E., Prince, R .C., Pickering, I. J., 1995. Mechanisms of cadmium mobility and accumulation in Indian mustard. Plant Physiol., 109: 1427-1443.

Wang,J.H., Luo,J.P., Zha, X.Q., Feng, B.J.,2010. Comparison of antitumor activities of different polysaccharide fractions from the stems of Dendrobium nobile Lindl, Carbohyd. Polym., 79, 1:114-118

Wang,L.Q., Wang,C.Q., Gao,X., Xu,N., Lin, L., Zhao,H.J., Jia,S.H., Jia, L.,2015. Purification, characterization and anti-aging capacity of mycelia zinc polysaccharide by Lentinus edodes SD-08. BMC Complem. Altern. M., 15(1):111-131.

Wang,P.P., Huang,Q., Chen,C., You,L.J., Liu,R.H., Luo,Z.G., Zhao,M.M., Fu, X.,2019.The chemical structure and biological activities of a novel polysaccharide obtained from Fructus Mori and its zinc derivative, J. Funct. Foods, 54,64-73.

Zhang, J. T.，Xu, M.，Han, K.，2011． Effect of salt stress on plant nutrition and physiology of tamato seedings．Acta Agriculturae Boreali-occidentali Sinica, 20 (2) ,128-133. DOI:10.1007/s10499-007-9164-4.

Zhang, J., Xu, Z.Q., Huang, H., Tian, J.M., Zhang, G.D. 2020. Characteristics and Speciation Analysis of Heavy Metals in Soils Around a Phosphate Mining Area in Mabian,Sichuan. Sichuan Environment, 39(2):9-15. DOI: 10. 14034 /j. cnki. schj. 2020. 02. 002.

Zhang,M., Zhao,H., Shen,Y., Wang,Y., Zhao,Z.M., Zhang,Y.,2020. Preparation, characterization and antioxidant activity evaluation in vitro of Fritillaria ussuriensis polysaccharide-zinc complex, Int. J. Biol. Macromol., 146, 462-474.

Zheng, L.,Liu, M.,Zhai, G.Y.,Ma, Z.,Wang, L.Q.,Jia, L.2015. Antioxidant and anti-ageing activities of mycelia zinc polysaccharide from Pholiota nameko SW-03. J. Food Agric., 95(15). 3117-3126. DOI:10.1002/jsfa.7048

Tables

Table 1 Effect of WUE of Dendrobium nobile leaves under different Zn treatment