2.3 Effects of zinc on the antioxidant system ofDendrobium nobile
The
content
of hydrogen peroxide
(H2O2)
was determined
according to the method of Park et
al. (Park et al.,2016). The
content
of superoxide anion (O2-) was
determined by using the method of Chen et al.( Chen et al.,2019). The
SOD activity was determined by using nitroblue tetrazolium (NBT)
photochemical reduction method(Milosevic et al., 1996).
Ascorbate
peroxidase (APX) activity was determined by ascorbate oxidation(Carolina
et al., 2017). The content of
AsA
was assayed according to Kunpatee et al. (Kunpatee et al.,2020) with
mg·g-1 FW as the unit of measurement. The content of
reduced glutathione (GSH) was determined by mercapto reagent(DTNB)
according to the method of Nagalakshmi (Nagalakshmi et al.,
2001).