Healthy 1-year-old seedlings with similarly growth rate were used in this research. Dendrobium nobile were cultivated with pine bark as substrate, 20 seedlings as a pot and 75% shading. After 50 days of planting, the plants were treated by different concentrations of zinc solution every 7 days for 3moths (the exudate was recycled and irrigated repeatedly until evenly mixed with the cultivated substrate). The test was performed without adding Zn²⁺ as a control group(CK), and the Zn²⁺ treatment groups were: T1 group (50 μmol/L), T2 group (100 μmol/L), T3 group (200 μmol/L), T4 group (400 μmol/L), T5 group (800 μmol/L), T6 group (1000 μmol/L), and T7 group (2000 μmol/L). After the end of treatment, the leaves and stems of the Dendrobium nobile with similar growth were selected to test on the 7th, 14th, and 21st days. On the 21st day, the roots ofDendrobium nobile were pulled out, which were washed, then dried in an oven for index determination. Each indicator was tested 3 times.