Gene mutation analysis.
Gene sequencing of Fms related tyrosine kinase 3 (FLT3)- internal tandem duplication (ITD) , FLT3- tyrosine kinase domain (TKD), c-KIT (exons 8 and 17) and NPM1 was performed by polymerase chain reaction (PCR) and direct Sanger sequencing according to our previous study [7,12].