Genotypic characterization of Mycobacterium bovisisolates from dairy cattle
Elizabeth Hortêncio de Melo 1*, Harrison
Magdinier Gomes2, Ícaro Rodrigues dos
Santos2, José Augusto Bastos
Afonso3, Marília Masello Junqueira
Franco4, Hélio Langoni4, Antonio
Carlos Paes4, Philip Noel Suffys2,
Carla Lopes de Mendonça3
Elizabeth Hortêncio de Melo 1. Corresponding
author: Elizabeth Hortêncio de Melo. Programa de Pós-Graduação em
Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom
Manuel de Medeiros, S/N, Dois Irmãos. 52171-900. Brasil, Pernambuco.
E-mail:
elishmelo@hotmail.com
Harrison Magdinier Gomes2. Laboratório de Biologia
Molecular Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fiocruz, Rio
de Janeiro, RJ, Brasil.
Ícaro Rodrigues dos Santos2. Laboratório de Biologia
Molecular Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fiocruz, Rio
de Janeiro, RJ, Brasil.
José Augusto Bastos Afonso3. Clínica de Bovinos,
Campus Garanhuns/UFRPE, Pernambuco, Brasil.
Marília Masello Junqueira Franco4. Departamento de
Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e
Zootecnia, UNESP, Botucatu, Brasil.
Helio Langoni4. Departamento de Higiene Veterinária e
Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, UNESP,
Botucatu, Brasil.
Antonio Carlos Paes4. Departamento de Higiene
Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e
Zootecnia, UNESP, Botucatu, Brasil.
Philip Noel Suffys2. Laboratório de Biologia Molecular
Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fiocruz, Rio de
Janeiro, RJ, Brasil.
Carla Lopes de Mendonça3. Clínica de Bovinos, Campus
Garanhuns/UFRPE, Pernambuco, Brasil.
ABSTRACT
Molecular diagnosis of bovine tuberculosis plays an essential role in
the epidemiological knowledge of the disease. Bovine tuberculosis caused
by Mycobacterium bovis represents a risk to human health. This
study aimed to perform the genotypic characterization of M. bovisisolated from bovines diagnosed as tuberculosis from dairy herds in the
state of Pernambuco, Brazil. Granulomas from 30 bovines were sent for
microbiological culture and colonies compatible withMycobacterium spp were obtained in at least one culture from
17/30 granulomas. All isolates were confirmed to be M.
tuberculosis bovis by spoligotyping and 24loci MIRU-VNTR
typing. While spoligotyping characterized the isolates as SB0121,
SB0295, SB0852, SB0120 and an unclassified genotype, 24lociMIRU-VNTR rendered two clusters of two isolates each and 13 unique
profiles. Loci ETR-A showed higher discriminatory power, andloci (ETR-B, ETR-C, MIRU16, MIRU27 and QUB26) showed moderate
allelic diversity. This is the first study on genetic variability of the
infectious agent cause of bovine TB in Pernambuco and demonstrates
variability of strains in the state. Thus, it corroborates the
importance of this microorganism as agent of bovine tuberculosis and its
zoonotic potential, being this epidemiological tool determinant in the
rigor of the sanitary practices of disease control in dairy herds.
Keywords : bovine tuberculosis, genotypic, Mycobacterium
bovis .