Analysis of MG-63 cellular metabolic status
Alterations in human osteoblast metabolic status, induced during internalization and persistence, were evaluated in the same experimental condition, by analysing the cell viability of MG-63 cells at 3h and 24h p.i. (Table 3).
At 3h p.i., MG-63 infected with ATCC12598 and ST228 strains showed a statistically significant increase in their metabolic activity compared to the control MG-63 uninfected cells, respectively, of +12.27% (p<0.001) and +7.57% (p<0.05), whereas the ST239 strain induced only a slight decrease in metabolic activity (-2.47%).
Comparing the cellular metabolic status of MG-63 infected with ST239vs ATCC12598, we found a statistically significant decrease (p<0.001), while no statistical difference was found comparing the toxicity of MG-63 cells infected with ST228 vs ATCC12598. A statistical difference in human cellular metabolic status was found by comparing MG-63 infected with ST239 and ST228 to each other (p<0.001) (Table 3A).
At 24h p.i., ATCC12598 and ST239 intracellular persistence induced a statistically significant decrease in metabolic activity of MG-63 cells, compared to the uninfected control cells, respectively, of -7.11% (p<0.05) and -18.32% (p<0.001), while the ST228 strain showed only a slight increase in metabolic activity (+3.79%).
Comparing the cellular metabolic status of MG-63 infected with ST239 and ST228 vs ATCC12598, we found a statistically significant decrease (p<0.001). Statistically significative differences in MG-63 metabolic status was found by comparing the results obtained from cells infected with ST239 and ST228 (p<0.001) (Table 3B).