2.2.3 Total soluble carbohydrate
Total soluble carbohydrate (TSC) was extracted thrice from 100 mg of
mature seed using extraction soluble including glacial acetic acid,
methanol, and water. It centrifuged at 3000 rpm for 10 min. The mixture
of 0.5 mL extraction with 0.5 mL of 5% (v: v) phenol solution and 2.5
mL purred sulfuric acid were heated at 90°C for 30 min (Dubois et al.,
1965). The absorbance was read at 490 nm. TSC content was recorded as
mg-1dry weight.