2.4 Statistical analysis
All data were analysed using GraphPad PrismTMsoftware. Normality testing was performed on all data using the Kolmogorov-Smirnov test. Statistical comparison of two means was performed using an unpaired two-tailed Student’s t -test should the data meet normality. A nonparametric Mann-Whitney U test was used if it did not. Data are presented as mean ± standard error of the mean (SEM). A p -value ≤ 0.05 was considered statistically significant.