2.4.3 Plant Area
The images of each plant area (PA) were determined by “ImageJ” Software program. In this program, the images had to be in a certain format (8-bit grayscale, 16-bit gray scale, etc.). Image-Type 8-bit option which is appropriate to S. hieraciifolia was selected and the scale was adjusted by entering the reference range 1 cm from the Analyze-Set Scale option. The area calculation for each shoot was performed from the Analyze-Tools-ROI Manager step.