Scorzonera hieraciifolia L. achenes were collected from Sivas
Tödürge Lake (İran-Turan Region, Turkey) and brought to the laboratory.Thellungiella parvula (synonym, Eutrema parvulum ) seeds
gently requested and acquired from Prof. Dr. İsmail TURKAN, Ege
University, İzmir, TURKEY. The mature achenes of S. hieraciifoliawere carefully separated from their pappus bristles. The achenes were
stratified for 2 d at 4°C in petri dishes containing double-layer wet
filter paper. After that, the achenes were treated with 10% (v/v)
sodium hypochlorite (NaOCl) solution for 8 min for sterilization,
followed by three rinsing stages with sterile distilled water.
To determine whether S. hieraciifolia is an extreme halophyte
plant, we compared S. hieraciifolia with the model plant for
extreme halophytes, T. parvula , closely related toArabidopsis thaliana . Similarly, T. parvula seeds were
treated with 10% (v/v) NaOCl solution for 4 min for sterilization
followed by three rinsing stages with sterile distilled water.