2.4.5 Photosynthetic Pigments
Photosynthetic pigments were determined according to Arnon (1949). The
sample of S. hieraciifolia (0.1 g) was homogenized in 5 ml of
80% acetone. The homogenate was centrifuged at 5000 g for 5 min at room
temperature. The absorbance of the supernatant was measured at 663, 645
and 450 nm by spectrophotometrically (Nicolet evolution 100, Thermo
Scientific, USA). The measurement of total chlorophyll (Chl) and
carotenoid (Car) contents were determined by the equations developed by
Lichtenthaler and Wellburn (1983).