Using dry extracts of the shoots, anion-cation analyzes were performed
with the Dionex ICS-5000 system (Haddad 1997). Analysis of chloride ion
content was performed with Dionex Ion Pac AS 9 HC (4x250 mm) separation
column and 10 mM sodium carbonate fluid phase (flow rate: 1 ml/min).
Analysis of the cation ion contents (Na+,
K+, Mg2+, Ca2+)
was carried out with Dionex Ion Pac CS 12 A (3x150 mm) column and 20 mM
meta sulfonic acid conductor phase (flow rate: 0.5 ml/min). Dionex CERS
500 (2 mm) was used as cation suppressor column and Dionex AERS 500 (4
mm) was used as anion suppressor column. Samples were given to the
column in 20 µl volumes by auto-sampler. Thermo Scientific conductivity
detector was used in the system and calculations were made with
Chromeleon Software System.