Protein extractions of SOD, CAT, glutathione peroxidase (GPX; E.C. 1.11.1.9) and GR were performed using 4X PEB (protein extraction buffer, AS08 300 Agrisera Inc.). Samples were dissolved in an equal volume of sample buffer (2X Laemmli sample buffer 1610737 Bio-Rad) and heated at 95 °C for 5 min. Separation of total proteins (30 μg) by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, (TGX Stain-Free Precast Protein Gels, 4568095 Bio-Rad) was performed by electrophoresis (Mini-PROTEAN Tetra Cell system, 165800 Bio- Rad) at room temperature. The marker (Precision Plus Protein Western C Blotting Standards, 1610376 Bio-Rad) was used to determine the molecular weight of the proteins. Proteins separated in gel electrophoresis were transferred to the PVDF membrane (Trans-Blot Turbo Mini PVDF Transfer Packs, 1704156 Bio-Rad) using the Trans-Blot Turbo Transfer System (1704155 Bio-Rad). After transfer, the membrane was blocked at 4 °C with 2.5% milk powder in TBS. Fe-SOD Chloroplastic Fe-Dependent Superoxide Dismutase (A S06 125) for SOD, Glutathione Peroxidase Chloroplastic (A S04 055) for GPX, and, Catalase (A S09 501) for CAT were primary used antibodies and they incubated overnight at 4 °C. After incubation, the membranes were incubated with goat anti-rabbit IgG-HRP secondary antibodies (AS09 602 Agrisera) for 4 h at room temperature. The density of the scanned protein bands was calculated with Image Lab Software (1709690, Bio-Rad).