Measurements of physicochemical properties
The physicochemical characteristics of soil and faeces samples were
measured as the Zhao et al reported (Q. Zhao et al., 2019). The
phyllosphere properties were measured by the similar methods after
ultrasonic leaf washing with 50MHz and buffer elution with potassium
phosphate buffer (Zhang et al., 2008). The gravimetric water content was
determined as the moisture with oven-drying at 105 ℃ for 24 h; the pH
was measured in the shaken soil water suspension with 1:2.5 soil: water
ratio; total organic carbon (TOC) of samples was measured by a TOC5000A
Analyzer (Shimadzu Corp., Kyoto, Japan) (Q. Zhao et al., 2019); total
phosphate (TP) was determined with the method as previous described
(Brookes, Powlson, & Jenkinson, 1982); total carbon (TC) and nitrogen
(TN) were analyzed with a Vario EL III Elemental Analyzer (Elementar,
Hanau, Germany); C/N was calculated by the ratio value of TC and TN;
NO3--N and
NH4+-N of samples were determined by
Continuous Flow Analyzer (AA3, SEAL analytical, Germany) (Z. B. Zhao et
al., 2019).