3.4 Determining the EC50 values of aprotinin for
various influenza A and B viruses
To further characterize the effects of aprotinin on various subtypes of
influenza A and B viruses, we determined its EC50 values
in MDCK cells against different influenza subtypes. We treated MDCK
cells infected with different influenza virus strains with varying
concentrations (10‒200 nM) of aprotinin (Fig. 6). Aprotinin inhibited
the infection of several influenza viral strains in a dose-dependent
manner. The calculated EC50 value for aprotinin against
each influenza strain is shown in Table 1.