3.4 Determining the EC50 values of aprotinin for various influenza A and B viruses
To further characterize the effects of aprotinin on various subtypes of influenza A and B viruses, we determined its EC50 values in MDCK cells against different influenza subtypes. We treated MDCK cells infected with different influenza virus strains with varying concentrations (10‒200 nM) of aprotinin (Fig. 6). Aprotinin inhibited the infection of several influenza viral strains in a dose-dependent manner. The calculated EC50 value for aprotinin against each influenza strain is shown in Table 1.