Plasma and right ventricle brain natriuretic peptide (BNP)
Plasma samples (1.0 mL) and homogenous right ventricle (RV) were expressed in v/v for TFA and homogenate in w/v and then centrifuged at 2000 × g for 15 min at 4°C. Enzyme-immunoassay kits (Phoenix Pharmaceuticals, Burlingame, CA, USA) were used to measure plasma and RV BNP contents according to the manufactural protocol.