2.1 │ Identification and processing of histone H1 subtypes interacting proteins
The partnering proteins of five standard human histone H1 somatic subtypes (H1.1 – H1.5) were found in the APID (Agile Proteins Interactomes DataServer) database. 9 APID contain the records of protein-protein interactions supported by detection methods evidencing a direct physical interaction between the protein pair, i.e. binary interactions, and identified by co-complex associations, i.e. indirect interactions. A type of binary interaction was derived from the records of BioGRID (Biological General Repository for Interaction Datasets) 10 and IntAct (Molecular Interaction Database) 11 databases, a literature curated sources of molecular interaction data. The sequences of selected proteins were searched in the resource of protein sequence and annotation data UniProt12 and further analyzed in the FASTA format.