2.1 │ Identification and processing of histone H1 subtypes
interacting proteins
The partnering proteins of five standard human histone H1 somatic
subtypes (H1.1 – H1.5) were found in the APID (Agile Proteins
Interactomes DataServer) database. 9 APID contain the
records of protein-protein interactions supported by detection methods
evidencing a direct physical interaction between the protein pair, i.e.
binary interactions, and identified by co-complex associations, i.e.
indirect interactions. A type of binary interaction was derived from the
records of BioGRID (Biological General Repository for Interaction
Datasets) 10 and IntAct (Molecular Interaction
Database) 11 databases, a literature curated sources
of molecular interaction data. The sequences of selected proteins were
searched in the resource of protein sequence and annotation data UniProt12 and further analyzed in the FASTA format.