2.9 Human serum Rickettsia-specific IgG antibody detection
The human Rickettsia-IgG ELISA KIT (Cat No: JL48935) of Jianglai Biotech Co., Ltd. was used for IgG antibody detection and the detection antibody type was Rickettsia IgG. Serum from healthy people and distilled water were used as negative and blank controls, respectively.
First, The concentration of the standard product is 100, 50, 25, 12.5, 6.25, 0 ng/mL in sequence, and 50 µL for each well. Next, Add sample diluent 40 µL on the Enzyme labeling plate first, and then add the sample to be tested 10 µL (the final dilution of the sample is 5 times). Add 100 µL enzyme reagents to each well, except for blank wells. The samples were incubated at 37 ℃ for 1 hour and then washed with washing solution,repeat 5 times,pat dry. Add color developer A 50 µL to each well, and then add color developer B 50 µL. Gently shake and mix well, and develop color at 37 ℃ in the dark for 15 minutes. Add stop solution 50 µL to each well to stop the reaction. 450nm wavelength measurement of absorbance of each hole, OD was measured after zero-adjusting the blank control reaction.