2.6 Amplification of SFTSV whole genome
Previously designed primers for amplification of S (3 primer sets), M (7 primer sets), and L (13 primer sets) segments were used for whole genome sequencing. Primers were synthesized by Sangon Biotech (Shanghai) Co., Ltd. The size of the target band amplified by each primer set for the S and M fragments was around 600 bp. Amplification conditions: 94 ℃, 5 min; 35 cycles: 94 ℃, 30 s; 55 ℃, 30 s; 72 ℃ 1 min; 72 ℃, 5 min; 30-µL system. The size of the target band amplified by each primer set for the L fragment was about 350‒650 bp. Amplification conditions: 94 ℃, 5 min; 35 cycles: 94 ℃, 30 s; 63 ℃, 30 s; 72 ℃, 1 min; 72 ℃, 5 min; 30-µL system.