2.9 Human serum Rickettsia-specific IgG antibody
detection
The human Rickettsia-IgG ELISA KIT (Cat No: JL48935) of Jianglai Biotech
Co., Ltd. was used for IgG antibody detection and the detection antibody
type was Rickettsia IgG. Serum from healthy people and distilled
water were used as negative and blank controls, respectively.
First,
The concentration of the standard product is 100, 50, 25, 12.5, 6.25, 0
ng/mL in sequence, and 50
µL
for each well. Next, Add sample diluent 40 µL on the Enzyme labeling
plate first, and then add the sample to be tested 10
µL (the final dilution of the sample
is 5 times). Add 100 µL enzyme reagents to each well, except for blank
wells. The samples were incubated at 37 ℃ for 1 hour and then washed
with washing solution,repeat 5 times,pat dry. Add color developer A 50
µL to each well, and then add color developer B
50
µL. Gently shake and mix well, and develop color at 37 ℃ in the dark for
15 minutes. Add stop solution 50 µL to each well to stop the reaction.
450nm wavelength measurement of absorbance of each hole, OD was measured
after zero-adjusting the blank control reaction.