2.4 SFTSV nucleic acid detection
Using highly conserved regions in SFTSV M as target regions, specific primers were designed for PCR amplification. The primers were MF3 (5’-GATGAGATGGTCCATGCTGATTCT-3’) and MR2 (5’-CTCATGGGGTGGAATGTCCTCAC-3’) with a target band size of 560 bp. Amplification conditions: 94 ℃, 5 min; 94 ℃, 30 s, 55 ℃ 30 s, 72 ℃ 1 min, 35 cycles; 72 ℃, 5 min; 30-µl system.