2.7 SFTSV whole genome sequencing and analysis
The PCR products were subjected to agarose gel electrophoresis, and the amplified products were sent to Sangon Biotech (Shanghai) Co., Ltd for sequencing. The sequences were spliced using the SeqMan software. GenBank was used as source of reported strain sequences, and DNASTAR and MEGA6.0 were used to conduct a comprehensive analysis of the three fragments of the SFTSV strain, to identify the virus genotype, and to analyze the genetic evolution of the strain. For paired and multiple alignments, the maximum likelihood method (ML) was used to build a phylogenetic tree based on the L, M, and S sequences. The bootstrap method with 1000 replications was used to estimate the reliability of the branches.