2.6. Expression analysis of LHCI and LHCII genes through RT-PCR
2.0 µl of cDNA from reverse transcription was used as template and LHCI
and LHCII genes specific primers were employed to amplify the desired
fragment through RT- PCR with optimized condition as initial
denaturation at 95 °C for 10 minutes, denaturation at 95 °C for 20
seconds, annealing at 58 °C for 40 seconds extension at 72 °C for 30
seconds by 34 cycles. The final product of RT-PCR was separated running
on 1% agrose gel by the process of electrophoresis and visualized on
alpha in notech gel documentation system.