Altered expression of PTPRT downstream target genes in the tumors from two carriers
In order to check the downstream consequences of the PTPRTmutation, a Taqman real-time qPCR was performed to evaluate the expression of PTPRT target genes BCL2-XL and SOCS3 in the tumors developed by family members II:6 and II:4. As shown in Figure 5A,BCL2-XL was significantly overexpressed in the colon tumor from member II:6 when compared to a healthy colon cDNA pool. In the same way, the breast tumor from member II:4 showed a consistent higherBCL2-XL expression than a healthy breast sample used as a control (Figure 5B). On the other hand, SOCS3 seemed to be considerably underexpressed in both tumors (CRC from II:6 and breast cancer from II:4), as shown in Figure 5C and D.