Figure 4. Allele-specific expression of wild-type and D1364Gfs*24 PTPRT.
(A) Digital PCR visualization of the allele-specific expression assay performed in the healthy colon tissue (top) and colorectal cancer (CRC) (bottom) of cc765 family member II:6. The FAM dye detects the mutant allele (circled with dashed line), while the VIC dye detects the wild-type allele (circled with solid line). B) Quantification of the allele-specific expression obtained by digital PCR presented as Target/Total, where ”Target” is the mutant PTPRT allele. Data was collected from two independent experiments, and the error bars correspond to the confidence intervals.