Reverse transcription PCR (RT-PCR)
RT-PCR was performed using the PrimeScript RT Reagent Kit (Perfect Real
Time, Takara, Clontech), following the kit’s instructions. The absence
of genomic DNA (gDNA) in cDNA samples was confirmed prior to their use
by a PCR targeting exons 2-3 of PALB2 , which allowed the
discrimination between gDNA and cDNA. The primers used for this test are
available upon request.