Fig5. In situ localization of
CD4+Foxp3+,
CD8+Foxp3+,
Foxp3+PD1 and Foxp3+PD-L1 inCC tissues(multiple fluorescence immunohistochemical staining).Representative images show tissue sections from CC with (A )
CD8+-opal650(magenta), Foxp3-opal570(pink), and
DAPI(4,6-diamidino-2-phenylindole; blue) labeling,
(B )CD4+-opal520(green), Foxp3-opal570(pink)
and DAPI(blue) labeling, (C )Foxp3-opal570(pink),
PD1-opal620(orange) and DAPI(blue) labeling, and
(D )Foxp3-opal570(pink), PD-L1-opal540(yellow) and DAPI(blue)
labeling. The graph show combined date for multiple fluorescence
immunohistochemical staining analysis of counts of
CD4+ /CD8+ T cell(E ),
CD4+PD1/CD8+PD1(F ) and
Foxp3+PD1/Foxp3+PD-L1(G )
expression on CC and healthy cervical tissues from the operation.
Images(A ), (B ), (C ) and (D ) are from
the same specimen and representative of CC patients. All images were
captured using an Evos FL Auto 2 cell imaging system(Thermo Fisher
Scientific) and merged using image J software. Data are reported as
mean±SEM.
*P<0.05;**P<0.01;***P<0.001;****P <0.0001(Student’s
t test).