Dispase-based dissociation assay
After incubation, confluent cell monolayers were washed with Hank´s
buffered saline solution (HBSS) and subjected to 2.4 U/ml dispase II
(Sigma Aldrich, USA) in HBSS for 20 min at 37 °C, 95 % humidity and 5
% CO2. After detachment of the monolayer the reaction
was stopped by adding 200 µl HBSS. Defined shear stress was applied with
an electrical pipette. Images for fragment counting were taken using a
binocular microscope (Leica, Germany) and an EOS 600D camera (Canon,
Japan).