2.7.6. Calcium assay
The calcium content assay is a direct colorimetric assay based on theĀ O-Cresolphtalein method (Baldino et al., 2015). The samples were washed with PBS and lysed by 0.6 M HCL. After addition of the reagent to the calcium solutions, based on the applied kit protocol (Pars Azmoon, Iran), the optical density (OD) of the samples was measured at 570 nm.