2.4. Genome sequencing
For single nucleotide polymorphism (SNP) discovery in thepho13 -negative strain (YSX3), the genome of the YSX3 strain and
its parental strain (L2612) were re-sequenced as described previously
(Kim et al., 2013d). Briefly, genomic DNA
was prepared by YeaStar Genomic DNA Kit (Zymo Research), and the DNA
quality was evaluated on a 1% agarose gel. The genomic DNA samples were
then sequenced using an Illumina HiSeq 2000 system, and the sequencing
results were analyzed using the CLC Genomic Workbench (version 6.5)
program. Among the 46 SNPs identified, 44 SNPs were found to be
non-synonymous, and only 2 SNPs in YEN1 and GCR2 genes
were confirmed in the YSX3 strain by Sanger sequencing. The two SNPs
were also confirmed.