2.3. Fermentation profiles
Cell growth was monitored at 600 nm using a spectrophotometer
(OD600). The concentrations of substrates and
metabolites were determined by high-performance liquid chromatography
(Agilent Technologies 1260 Series, Santa Clara, CA, USA) equipped with a
refractive index detector using a Rezex ROA-Organic Acid
H+ (8%) column (Phenomenex, Inc., Torrance, CA, USA).
The column was eluted with 0.005 N
H2SO4 at a flow rate of 0.6 mL/min and
50°C.