Genome-wide association study (GWAS)
Genome-wide association analysis of recurrent wheeze and asthma
diagnosis used a logistic regression model, adjusted for sex and three
significant PCs using Plink 1.9.(23) All variants with minor allele
frequencies (MAF) less than 1% in the population were filtered out due
to the lack of statistical power for detecting associations with rare
variants. For significantly associated SNVs, local plots of
recombination rates were visualized using LDassoc function of the LDlink
web application.(30) Manhattan plots and quantile-quantile (qq) plots of
GWAS analysis were generated using the qqman package in R.(31)