Nucleotide sequencing and sequence analysis
PCR products were purified using the Gene Jet PCR purification kit;
Fermentas (Thermo Fisher Scientific). Each purified amplicon was
sequenced in both forward and reverse directions using the amplification
primers (Table 1). Amplicons were sequenced in an automated sequencer
(Macrogen Company 24, Gasan-dong, Geumchun-gu, Seoul 153-781, Korea).
Sequence data similarity searches were analyzed by using NCBI-BLAST
programme (http://www.ncbi.nlm.nih.gov/BLAST). The comparisons of
obtained nucleotide sequences and their multiple alignments were
performed using the BioEdit sequence alignment editor (CLUSTALX software
version 7.0.9.0) (6/27/07) for multiple sequence alignment (Figure S1).
Sequences were then submitted to NCBI GenBank using BankIt
(http://www.ncbi.nlm.nih.gov/WebSub/?tool=genbank) under the
accession numbers of: