Mycoplasma bovis typing
Positive M. bovis  isolates were further confirmed by PCR reaction. The PCR conditions for this reaction were denaturation at 94oC for 45 seconds, primer annealing at 60oC for 1 min., and extension at 72oC for 2 min. The reactions were performed for 30 or 35 cycles with a final extention at 72oC for 3 min.