Mycoplasma bovis typing
Positive M. bovis isolates were further confirmed by PCR
reaction. The PCR conditions for this reaction were denaturation at
94oC for 45 seconds, primer annealing at
60oC for 1 min., and extension at
72oC for 2 min. The reactions were performed for 30 or
35 cycles with a final extention at 72oC for 3 min.