Detection of haemolytic activities of M. bovis
The effect of M. bovis on RBCs was tested in a liquid environment. In this assay, the RBCs formed a loose pellet. β-hemolysis would result in the disruption of the erythrocytes and the release of the red haemoglobin. We observed, however, that the erythrocytes remained intact, but noted a colour change of the blood cells from red to brown which is characteristic for α-haemolysis resulting from a modification of haemoglobin thus they were phenotypically haemolytic.