Antibiotic susceptibility test
The 13 M. bovis isolates were tested for susceptibility to eight
antimicrobial agents by the disc diffusion method. The antimicrobial
agents used in this study included: Fluoroquinolone:ciprofloxacin (5 ug); Macrolides: spiramycin (100 ug), tylosin
(30 ug) and erythromycin (15 ug); Phenicol: florfenicol (30
ug); Aminoglycoside: streptomycin (10ug); Lincosamide:lincomycin (2 ug); Tetracycline: doxycycline (30 ug) according
to the procedures outlined in CLSI (2012). The type strain M.
bovis ATCC 25523/ PG45 was used as quality control
Molecular
Identification