Determination of in vitro hydrogen sulphide production
The H2S produced by the 13 M. bovis isolates was
determined using lead acetate detection strips as previously described
(Großhennig et al.,2016). Briefly, growing cells were harvested and
resuspended in 1ml 1x PBS (pH 7.4) supplemented with L-cysteine. Sterile
filter paper strips impregnated with lead acetate were affixed to the
inner wall of the test tubes above the level of the liquid culture. An
aliquot without L-cysteine served as the control. After 18 h of
incubation at 37oC, the test strips were read and
photographed.