Nucleotide sequencing and sequence analysis
PCR products were purified using the Gene Jet PCR purification kit; Fermentas (Thermo Fisher Scientific). Each purified amplicon was sequenced in both forward and reverse directions using the amplification primers (Table 1). Amplicons were sequenced in an automated sequencer (Macrogen Company 24, Gasan-dong, Geumchun-gu, Seoul 153-781, Korea). Sequence data similarity searches were analyzed by using NCBI-BLAST programme (http://www.ncbi.nlm.nih.gov/BLAST). The comparisons of obtained nucleotide sequences and their multiple alignments were performed using the BioEdit sequence alignment editor (CLUSTALX software version 7.0.9.0) (6/27/07) for multiple sequence alignment (Figure S1). Sequences were then submitted to NCBI GenBank using BankIt (http://www.ncbi.nlm.nih.gov/WebSub/?tool=genbank) under the accession numbers of: