16S rRNA identification of Mycoplasma species usingMycoplasma group-specific primers set
The same procedure for 16S rRNA PCR reaction as described above was used for Mycoplasma species identification, but using the following PCR conditions: 40 cycles of denaturation at 94oC for one minute, annealing at 55oC for 1 min and extension at 72oC for 2 min.