Ebselen diminishes CS-induced vascular oxidative stress
and downregulation of eNOS
We next sought to define the underlying mechanism by which ebselen
prevented CS-induced endothelial dysfunction. Mice chronically exposed
to CS had increased vascular endothelial oxidative stress as measured by
3-NT expression (Figure 6, p <0.05). However, ebselen
administration significantly reduced CS-induced increases in 3-NT
expression to baseline sham levels (Figure 6, p <0.05).
Moreover, ebselen treatment completely prevented the downregulation of
eNOS as a result of CS-exposure (Figure 7, p <0.05).
Collectively, these findings suggest that ebselen reduced CS-induced
vascular oxidative stress and subsequently prevented the downregulation
of eNOS within the thoracic aorta.