Data and Statistical analysis
All data and statistical analysis comply with the recommendations on experimental design and analysis in pharmacology (Curtis et al, 2018). All data presented as mean ± standard error of the mean (SEM) unless otherwise stated. Statistical differences between treatments were determined by analysis of variance (ANOVA) followed by Tukey’s multiple comparison post-hoc tests where appropriate. One-way ANOVA were used for three of more unmatched groups. Two-way ANOVA were used to analyse data when response was influenced by two categorized factors of interest. For comparisons between two groups of results, regular unpaired t-tests were used to generate the P value. All statistical analyses were performed using GraphPad PrismTM for Microsoft Windows® (Versions 8, Graphpad software®, USA) where p <0.05 was accepted as significant for all cases.