2.2.4. 1H-NMR analysis
Prepared MO and EMO were characterized by 1HNMR
technique using 5 mm NMR tube. 1H NMR spectra were
recorded in CDCl3 employing TMS as an internal chemical
shift reference with the following acquisition parameters: 45° pulse
angle of 4.87 μs pulse width; transmitter frequency offset (O1P) of 5
ppm; relaxation delay (d1) time 4 s; acquisition time 2.18 s; spectral
width 8.75 ppm, 64 scans; spectral acquisition temperature 291–298 K.
All spectra were analyzed by the MestReNova package (6.0.2-5475). To
integrate the proton signals, the methoxy protons
(-OCH3) signal of the MO appearing at 3.63 ppm has been
assigned to 3 protons. In other words, the MO peak at 3.63 ppm is acting
as an internal standard.