3.4.3 Comparison with HPLC results
In order to compare the results obtained from the qHNMR technique, the
prepared standard samples with different MO and EMO molar ratios were
analyzed by an HPLC technique. In the HPLC chromatogram, the retention
time of MO and EMO were found to be 7.92 and 8.38 minutes, respectively.
In the HPLC chromatogram of standard mixtures of MO/EMO, percentage peak
area values were calculated (Table 1 ) and was found to be
directly proportional to the percentage molar concentration of the
compounds (Fig 2s –5s , supporting information). The
results of EMO molar concentration obtained from HPLC when correlated
with that obtained from the qHNMR technique showed acceptable agreements
affirming the validation of the qHNMR method (Fig. 5 ).