4. Conclusion:
In this study, qHNMR was used to quantify epoxy methyl oleate prepared from oleic acid. Two different 1H NMR equations have been proposed for the quantification of EMO in EMO and MO mixture. The EMO quantification values obtained from equation 2 was found to demonstrate better correlation (0.999) with that of EMO present in the standard mixture of EMO and MO. The results obtained from the1H NMR technique are also in accordance with that of HPLC (R2 = 0.996). The developed equation 2has also been applied on the quantification of the real EMO sample obtained from the epoxidation of methyl oleate. The linearity, reproducibility and validity of the qHNMR technique suggest that qHNMR could be used as a reliable and quick method for the quantification of epoxy methyl oleate (EMO).