3.4.2 Reproducibility and Linearity:
A linearity curve for both the derived equations was also evaluated
(Fig. 4 ) which showed the correlation coefficient of
> 0.999. To test the reproducibility and linearity of the
method, three different technical hands have followed the identical
experimental procedure to quantify the EMO in the mixture of MO/EMO and
employed equation 1 and 2 . The observed results showed
good correlation with the true concentrations confirming the excellent
reproducibility of the method (Table 1a, supporting
information). As shown in Table 1 , as the EMO concentration in
the mixture increases beyond 70%, both the equations were found to be
consistent with the actual values. Nevertheless, equation 2 was
found to be more consistent with actual concentration as well as with
HPLC analysis at all concentration levels.