Mycobacterium inoculation on Lowenstein-Jensen medium (LJ Media)
Decontaminated samples 5-10ml were transferred to follicle tube.
Inoculated in to two slants of LJ media for liquid medium and smear
microscopic examination. Using a pipette, 3-4 drops (0.2-0.4 ml) were
inoculated in each LJ slant. All LJ slants were incubated at 37°C and
examined daily. After formation of mycobacterium colonies, ZN staining
was carried out to identify AFB, while samples were considered culture
negative if there were no colonies formed after 8 weeks of incubation
time [14].