Mycobacterium inoculation on liquid medium (MGIT)
Decontaminated samples 3-4 drops (0.5ml) through pipette inoculated in to MGIT. The MGIT tubes were kept in BACTEC 960 for scanning purpose. The tubes were incubated at 37°C and examine daily up to eight weeks. When mycobacterium growth appears in MGIT tube, automated mycobacterial detection system of BACTEC 960 indicated the MGIT tube with the help of green signal [14].