GeneXpert assays
The GeneXpert MTB/RIF test was performed using GeneXpert Model GX-XVI GXXVI-16-LXX as per the manufacturer’s instruction. The decontaminated samples were kept for 15 minutes at rest. After 15 minutes pulmonary samples were centrifuged for 5 minutes at 1000 relative centrifugal force (RCF), while extra pulmonary sample was centrifuge for 15 minutes at 3000 rcf. Through pipet 2.5 ml sample was taken to cartridge and placed in modules of GeneXpert. GeneXpert scans the cartridge, required 1 hour and 52 minutes for detection.