Results
Screening of plant
extracts for their potential sPLA2 inhibitory activity
We initially compared the sPLA2 inhibitory activities of the different
extracts of T. hispida (THA, THE and THB), C.rotundus (CRA
and CRE) and J.adathoda (JAA and JAE) with that of a commercial
sPLA2 inhibitor (CAY 10590) on bee venom. Bee venom is known to have
high levels of sPLA2 IIA. The inhibitory activity of each extract at
different concentrations on bee venom sPLA2 is shown in table 1. We
found that the extracts of T. hispida (THA and THE), showed the
highest sPLA2 activity, which was comparable to the inhibition levels of
CAY 10590. Of the T. hispida extracts, THB showed the highest
level of inhibition of the sPLA2 activity and therefore, we proceeded
with further analysis of this extract.