Thin layer Chromatography (TLC) of THB
In the assays as the highest sPLA2 inhibitory activity was seen with THB, further characterization by TLC was only carried out on the THB fraction. THB was subject to thin layer chromatography on silica (Merck, EMD Millipore GF 254) using ethyl acetate: methanol: water (8: 1.2:1 v/v/v) as the developing solvent. The developed TLC was sprayed with Natural Products/ polyethylene glycol reagent as the visualizing agent.