Assays for measuring secretory phospholipase A2 (sPLA2) activity and sPLA2 inhibitors
The sPLA2 activity was determined by a commercial assay (Abcam, UK), containing bee venom as the positive control (Catalog no: ab133089). The assay was carried out and interpreted according to the manufacturer’s instructions. All assays were carried out in triplicate. The sPLA2 activity was expressed as nmolmin-1mL-1. The sPLA2 inhibitory activity of plant extracts was compared with the commercially available sPLA2 inhibitor, CAY10590 (Cayman, USA).