2.1 | Animals
All animal care and study protocols complied with the guidelines of the
ethical use of animals and were approved by the Animal Care and Use
Committee (Third Military Medical University, Chongqing, China). All
efforts were made to minimize animal suffering and reduce the number of
animals used. Animal studies are reported in compliance with the ARRIVE
guidelines and with the recommendations made by the British
Journal of Pharmacology (BJP) (Kilkenny et al., 2010; McGrath &
Lilley, 2015). Experimental protocols and design adhere to BJPguidelines (Curtis et al., 2015).
Mice were bred and housed in the laboratory animal room at Chongqing
University under specific pathogen-free conditions. Mice were kept in
open polypropylene cages with clean chip bedding. The animal room was
maintained at a controlled temperature (22 ± 3 °C) and a 12 hr cycle of
light and dark. Five mice in each cage were free to drink water and were
fed on a regular diet (0.3–0.8% sodium, 1.0–1.8% calcium, 0.6–1.2%
phosphorus, Beijing Keao Xieli Feed Corporation, China) unless otherwise
indicated. To obtain tissues, all mice were killed by i.p. injection
with 2,2,2-tribromoethanol (250 mg·kg−1,
Sigma-Aldrich, Cat# T48402).