Mycobacterium tuberculosis isolates
All 110 MTB clinical isolates collected (January 2018-March 2020) in the
province of Colon, Panama, were selected. A sample of clinical isolates
from the province of Chiriquí was obtained from Hospital Obaldía
(Panama) as the comparison group. Isolates were cultured on
Löwenstein–Jensen medium and DNA extracted using the cetyl
trimethylammonium bromide method (van
Soolingen, Hermans, de Haas, Soll, & van Embden, 1991).