Figure 1. The phylogeny and geographic distribution of sampled stickleback populations. A. A rooted neighbor-joining tree of stickleback populations based on Fst calculated from SNPs obtained via ddRAD sequencing (Stuart et al. 2017). Site IDs are in parentheses. Note that Sayward Estuary (SAY), Pye Outlet (PYO), and Campbell River Marsh (CRM) are estuary habitat, and the rest are freshwater habitat. B. The distribution of sampling sites on Vancouver Island, Canada overlaid on Google Map. The lower panel is an enlarged view of the rectangle box in the upper panel. Each site is represented by a circle. The color of the circles is consistent with the tip label circles in A.