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\begin{document}

\title{The correlation between methylenetetrahydrofolate reductase gene
polymorphism and high-risk pregnancy:a retrospective cohort study}



\author[1]{Jiahui Shen}%
\author[1]{Yanhui Jiang}%
\author[1]{Jie Liu}%
\author[1]{Le Chen}%
\author[1]{Yaochao Yang}%
\author[1]{Xiaoe Zeng}%
\author[1]{Qiwen Yuan}%
\affil[1]{Fifth Affiliated Hospital of Sun Yat-sen University}%


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  \date{May 08, 2024}


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\begin{abstract}
Objective: To explore the correlation between methylenetetrahydrofolate
reductase (MTHFR) 677C-T polymorphism and high-risk pregnancy(HRP), and
to establish a useful HRP management model during the perinatal period.
Design:Retrospective cohort study Setting:The Fifth Hospital of Sun
Yat-sen University Population:All women gave birth in the obstetrics
departmen between 2018 and 2020. Methods:Using polymerase chain reaction
and restriction fragment length polymorphism analysis (PCRRFLP) method
to detect MTHFR677C-T genotype. Screening is base on whether the
maternal has diagnosis HRP and have a regular antenatal~care. The
correlation between HRP and MTHFR677C-T polymorphism explained by
chi-square test and the logistic regression. Main Outcome Measure: The
alleles and genotypes of MTHFR 677C-T and the prevalence and
classification of HRP. Results:A total of 2,372 were eventually
enrolled, including 584 cases in the HRP group and 737 cases in the
control group. The MTHFR 677C-T allele was in Hardy- Weinberg
equilibrium(P\textgreater{}0.05). The MTHFR 677C-T polymorphism and
allele (T) were associated with HRP (P\textless{}0.05).To~be~specific,
they associated with Hypertensive disorders of Pregnancy(HDP), Pregnancy
with thyroid disease, gestational anemia and Preterm
birth(PTB)(P\textless{}0.05). After adjusting for confounders, the
mutant genotype (C/T+T/T) remained a susceptibility factor for HDP and
PTB(P\textless{}0.05). The allele (T) was associated with HDP,
Gestational diabetes mellitus and PTB(P\textless{}0.05). Conclusions:The
MTHFR 677C-T polymorphism was associated with HRP, especially HDP and
PTB. It is necessary to establish a useful model to manage the women
during the perinatal period. Funding: None Keywords:high-risk pregnancy,
MTHFR677C-T, pregnancy comorbidities. Tweetable abstract:MTHFR 677C-T
polymorphism is associated with high-risk pregnancy.%
\end{abstract}%



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