Data and statistical analysis
IC50 and EC50 values were determined by
non-linear regression using GraphPad Prism 8, which was also used for
statistical calculation. Sigmoid curves were fitted logistically with a
Hill slope of 1 or -1 for the activation and binding curve,
respectively.
To calculate the total density of receptors expressed
(Bmax) we used equation (1) (Deblasi, Reilly et al.
1989):